Use of cellular exosomes as a new carrier in breast cancer gene therapy

Posted: 2021-10-15 19:00:00
Background: Breast cancer is recognized as a major clinical challenge in gynecological diseases worldwide. Exosomes are small vesicles derived from multicellular bodies that are secreted by many cells into the extracellular environment and thus participate in intercellular communication through the transfer of genetic information such as encoded and non-encoded RNAs to target cells. Tumor-derived exosomes are thought to be a rich source of microRNAs (miRs) that can regulate the function of other cancer cells in the tumor microenvironment. However, the exact mechanisms by which tumor cell-derived exosomes affect their neighboring cells, as well as the bio-logical function of exosomal miRs in receptor cells, are not well understood. Materials and methods: In this study, after the overexpression of MiR-205 in breast cancer cells (MDA-MB-231 class), cell-derived exosomes were successfully isolated and characterized by electron microscopy and dynamic light scattering. Results: The determination of MiR-205 expression levels in exosomes secreted from engineered cells confirmed the high expression of this miR in exosomes. It was also found that the treatment of tumor exosomes carrying this miR had an apoptotic induction effect and also had a significant effect on reducing the expression of Bcl-2 gene transcript in a time-dependent manner in breast cancer cells (P < 0.001). Conclusion: Overall, this study suggests that exosomal transfer of tumor suppressor miRs to cancer cells could be a suitable platform for nucleic acid transfer to these cells and be highly effective in cancer treatment. Keywords: Exosomes; apoptosis; breast cancer; exozomy; gene therapy; microRNA; microRNAs.

参考サイト PubMed: exsome


9月 02, 2020 バイオアソシエイツ

GTPase Rab11aエクソソームが癌の成長と治療抵抗性を促進すると新研究が示唆

英国の助成を受けたオックスフォード大学の研究者による共同研究では、癌細胞が成長し治療によるストレスに癌細胞が適応する新しいメカニズムが明らかになった。 このメカニズムには、細胞がエクソソームとして知られている小さな小胞を放出することが含まれる。 これらの エクソソーム は、タンパク質、RNA、および他の分子の複雑な混合物を含み、周囲の細胞を再プログラムすることができる。エクソソームは、体内のすべての細胞によって放出され、免疫や生殖などの健康な個人の多くのプロセスで重要な役割を果たすと考えられている。…

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