ライフサイエンス研究メディア

バイオマーケットjpはバイオ・ライフサイエンス研究に役立つ情報を発信するWebメディアです

The proteomes of endometrial stromal cell-derived extracellular vesicles following a decidualising stimulus define the cells' potential for decidualisation success


Posted: 2021-09-15 19:00:00
Adequate endometrial stromal cell (ESC) decidualisation is vital for endometrial health. Given the importance of extracellular vesicles (EVs) in intercellular communication, we investigated how their protein landscape is reprogrammed and dysregulated during decidual response. Small EVs (sEVs) from human ESC-conditioned media at day-2 and -14 following decidual stimuli, were grouped as well- (WD) or poorly-decidualised (PD) based on their prolactin secretion and subjected to mass spectrometry-based quantitative proteomics. On day 2, in PD- versus WD-ESC-sEVs, 17 sEV- proteins were down-regulated (C5, C6; complement/coagulation cascades, and SERPING1, HRG; platelet degranulation and fibrinolysis) and 39 up-regulated (FLNA, COL1A1; focal adhesion, ENO1, PKM; glycolysis/gluconeogenesis, and RAP1B, MSN; leukocyte transendothelial migration). On day 14, in PD- versus WD-ESC-sEVs, FLNA was down-regulated while 21 proteins were up-regulated involved in complement/coagulation cascades (C3, C6), platelet degranulation (SERPINA4, ITIH4), B-cell receptor signaling and innate immune response (immunoglobulins). Changes from day 2 to 14 suggested a subsequent response in PD-ESC-sEVs with 89 differentially expressed proteins mostly involved in complement and coagulation cascades (C3, C6, C5), but no change in WD-ESC-sEVs ESC. Poor decidualisation was also associated with loss of crucial sEV-proteins for cell adhesion and invasion (ITGA5, PFN1), glycolysis (ALDOA, PGK1) and cytoskeletal reorganization (VCL, RAC1). Overall, this study indicates varied ESC response even prior to decidualisation, and provides insight into sEVs-proteomes as a benchmark of well decidualised ESC. It shows distinct variation in sEV-protein composition depending on the ESC decidual response that is critical for embryo implantation, enabling and limiting trophoblast invasion during placentation and sensing a healthy embryo. Keywords: complement/coagulation cascades; decidualised endometrial stromal cells; inter-cellular communication; proteomics; small extracellular vesicles.

参考サイト PubMed: exsome



バイオクイックニュース日本語版:エクソソーム特集

バイオクイックニュース日本語版
2月 22, 2021 バイオアソシエイツ

タウタンパク質の『もつれ』がアルツハイマー病の脳でどのように発達するのかが明らかに。

オーストラリア のクイーンズランド大学(UQ)の研究者は、認知症やアルツハイマー病の原因となる可能性がある脳細胞の新しい「播種」プロセスを発見した。 UQのクイーンズランド脳研究所の認知症研究者であるJürgen Götz…

ゲスト 864人 と メンバー 6人 がオンラインです