The Nma1 protein promotes long distance transport mediated by early endosomes in Ustilago maydis


Posted: 2021-11-24 20:00:00
Mol Microbiol . 2021 Nov 24. doi: 10.1111/mmi.14851. Online ahead of print. Affiliations Expand Affiliations 1 Karlsruhe Institute of Technology, Institute of Applied Biosciences, Department of Genetics, Fritz Haber Weg 4, 76131, Karlsruhe, Germany. 2 Department of Molecular Microbiology and Genetics and Göttingen Center for Molecular Biosciences (GZMB), University of Göttingen, Göttingen, Germany. Item in Clipboard Karina Schneider et al. Mol Microbiol. 2021. Show details Display options Display options Format Mol Microbiol . 2021 Nov 24. doi: 10.1111/mmi.14851. Online ahead of print. Affiliations 1 Karlsruhe Institute of Technology, Institute of Applied Biosciences, Department of Genetics, Fritz Haber Weg 4, 76131, Karlsruhe, Germany. 2 Department of Molecular Microbiology and Genetics and Göttingen Center for Molecular Biosciences (GZMB), University of Göttingen, Göttingen, Germany. Item in Clipboard CiteDisplay options Display options Format Abstract Early endosomes (EEs) are part of the endocytic transport pathway and resemble the earliest class of transport vesicles between the internalization of extracellular material, their cellular distribution or vacuolar degradation. In filamentous fungi, EEs fulfill important functions in long distance transport of cargoes as mRNAs, ribosomes and peroxisomes. Formation and maturation of early endosomes is controlled by the specific membrane-bound Rab-GTPase Rab5 and tethering complexes as CORVET (class C core vacuole/endosome tethering). In the basidiomycete Ustilago maydis, Rab5a is the prominent GTPase to recruit CORVET to EEs; in rab5a deletion strains, this function is maintained by the second EE-associated GTPase Rab5b. The tethering- and core- subunits of CORVET are essential, buttressing a central role for EE transport in U. maydis. The function of EEs in long distance transport is supported by the Nma1 protein that interacts with the Vps3 subunit of CORVET. The interaction stabilizes the binding of Vps3 to the CORVET core complex that is recruited to Rab5a via Vps8. Deletion of nma1 leads to a significantly reduced number of EEs, and an increased conversion rate of EEs to late endosomes. Thus, Nma1 modulates the lifespan of EEs to ensure their availability for the various long distance transport processes. Keywords: CORVET; Rab-GTPases; early endosomes; filamentous fungi; vesicle transport. This article is protected by copyright. All rights reserved. [x] Cite Copy Format: Send To [x]

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バイオクイックニュース日本語版:エクソソーム特集

バイオクイックニュース日本語版
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