Studying the Tumor Microenvironment in Zebrafish


Posted: 2021-10-19 19:00:00
Adv Exp Med Biol . 2021;1329:69-92. doi: 10.1007/978-3-030-73119-9_4. Affiliations Expand Affiliations 1 St. Anna Children's Cancer Research Institute, Innovative Cancer Models, Vienna, Austria. 2 Division of Anatomy, Department of Surgery, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada. 3 St. Anna Children's Cancer Research Institute, Innovative Cancer Models, Vienna, Austria. martin.distel@ccri.at. Item in Clipboard Caterina Sturtzel et al. Adv Exp Med Biol. 2021. Show details Display options Display options Format Adv Exp Med Biol . 2021;1329:69-92. doi: 10.1007/978-3-030-73119-9_4. Affiliations 1 St. Anna Children's Cancer Research Institute, Innovative Cancer Models, Vienna, Austria. 2 Division of Anatomy, Department of Surgery, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada. 3 St. Anna Children's Cancer Research Institute, Innovative Cancer Models, Vienna, Austria. martin.distel@ccri.at. Item in Clipboard CiteDisplay options Display options Format Abstract The tumor microenvironment significantly contributes to tumor initiation, progression, neo-angiogenesis, and metastasis, and a better understanding of the role of the different cellular players would facilitate the development of novel therapeutic strategies for cancer treatment. Towards this goal, intravital imaging is a powerful method to unravel interaction partners of tumor cells. Among vertebrate model organisms, zebrafish is uniquely suited for in vivo imaging studies. In recent years zebrafish has also become a valuable model in cancer research. In this chapter, we will summarize, how zebrafish has been used to characterize cells of the tumor microenvironment. We will cover both genetically engineered cancer models and xenograft models in zebrafish. The majority of work has been done on the role of innate immune cells and their role during tumor initiation and metastasis, but we will also cover studies focusing on adipocytes, fibroblasts, and endothelial cells. Taken together, we will highlight the versatile use of the zebrafish model for in vivo tumor microenvironment studies. Keywords: Adipocytes; Cancer; Compound testing; Extracellular vesicles; Fibroblasts; In vivo; Innate immune cells; Live imaging; Macrophages; Metastasis; Microglia; Neo-angiogenesis; Neutrophils; Tumor microenvironment; Tumor proliferation; Zebrafish. © 2021. The Author(s), under exclusive license to Springer Nature Switzerland AG. References Hanahan D, Weinberg RA (2000) The hallmarks of cancer. Cell 100(1):57–70 - PubMed - PMC - DOI Kienast Y et al (2010) Real-time imaging reveals the single steps of brain metastasis formation. Nat Med 16(1):116–122 - PubMed - DOI Harney AS et al (2015) Real-time imaging reveals local, transient vascular permeability, and tumor cell Intravasation stimulated by TIE2hi macrophage-derived VEGFA. Cancer Discov 5(9):932–943 - PubMed - PMC - DOI Streisinger G et al (1981) Production of clones of homozygous diploid zebra fish (Brachydanio rerio). Nature 291(5813):293–296 - PubMed - DOI Driever W et al (1996) A genetic screen for mutations affecting embryogenesis in zebrafish. Development 123:37–46 - PubMed - DOI Show all 98 references [x] Cite Copy Format: Send To [x]

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