SnRNA sequencing defines signaling by RBC-derived extracellular vesicles in the murine heart

Posted: 2021-10-19 19:00:00
Extracellular vesicles (EVs) mediate intercellular signaling by transferring their cargo to recipient cells, but the functional consequences of signaling are not fully appreciated. RBC-derived EVs are abundant in circulation and have been implicated in regulating immune responses. Here, we use a transgenic mouse model for fluorescence-based mapping of RBC-EV recipient cells to assess the role of this intercellular signaling mechanism in heart disease. Using fluorescent-based mapping, we detected an increase in RBC-EV-targeted cardiomyocytes in a murine model of ischemic heart failure. Single cell nuclear RNA sequencing of the heart revealed a complex landscape of cardiac cells targeted by RBC-EVs, with enrichment of genes implicated in cell proliferation and stress signaling pathways compared with non-targeted cells. Correspondingly, cardiomyocytes targeted by RBC-EVs more frequently express cellular markers of DNA synthesis, suggesting the functional significance of EV-mediated signaling. In conclusion, our mouse model for mapping of EV-recipient cells reveals a complex cellular network of RBC-EV-mediated intercellular communication in ischemic heart failure and suggests a functional role for this mode of intercellular signaling.

参考サイト PubMed: exsome

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12月 04, 2018 バイオアソシエイツ


細菌は、私たちが呼吸するあらゆる空気の中に存在する。気道がこれらの細菌の感染からどのように保護されるのか、今まで謎のままだった。細菌を吸入すると、細菌を直接攻撃する細胞から直ちに エクソソーム が分泌され、鼻の前部から気道に沿って抗菌タンパク質を送り、細菌が体の奥に入る前に防御する。 マサチューセッツEye and Earの研究チームは、2018年11月12日にJournal of Allergy and Clinical…

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