Reassessment of the proteomic composition and function of extracellular vesicles in the seminal plasma


Posted: 2021-10-14 19:00:00
Endocrinology . 2021 Oct 14;bqab214. doi: 10.1210/endocr/bqab214. Online ahead of print. Hanshu Wang 1 , Yu Zhu 1 , Chunhua Tang 1 , Zhiyang Zhou 1 , Zhengquan Wang 1 , Zhenhua Li 1 , Xiaoguo Zheng 1 , Shitao Chen 1 , Yuchuan Zhou 1 , Ajuan Liang 2 , Yanquan Li 1 , Yu Lin 1 , Fei Sun 1 3 4 Affiliations Expand Affiliations 1 International Peace Maternity & Child Health Hospital, Shanghai Municipal Key Clinical Specialty, Institute of Embryo-Fetal Original Adult Disease, School of Medicine, Shanghai Jiao Tong University, Shanghai 200030, China. 2 Department of Gynecology and Obstetrics, Center for Reproductive Medicine, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, China. 3 Institute of Reproductive Medicine, School of Medicine, Nantong University, Nantong, Jiangsu 226001, China. 4 Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, School of Basic Medicine, Ningxia Medical University, Yinchuan, 750004, China. Item in Clipboard Hanshu Wang et al. Endocrinology. 2021. Show details Display options Display options Format Endocrinology . 2021 Oct 14;bqab214. doi: 10.1210/endocr/bqab214. Online ahead of print. Authors Hanshu Wang 1 , Yu Zhu 1 , Chunhua Tang 1 , Zhiyang Zhou 1 , Zhengquan Wang 1 , Zhenhua Li 1 , Xiaoguo Zheng 1 , Shitao Chen 1 , Yuchuan Zhou 1 , Ajuan Liang 2 , Yanquan Li 1 , Yu Lin 1 , Fei Sun 1 3 4 Affiliations 1 International Peace Maternity & Child Health Hospital, Shanghai Municipal Key Clinical Specialty, Institute of Embryo-Fetal Original Adult Disease, School of Medicine, Shanghai Jiao Tong University, Shanghai 200030, China. 2 Department of Gynecology and Obstetrics, Center for Reproductive Medicine, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, China. 3 Institute of Reproductive Medicine, School of Medicine, Nantong University, Nantong, Jiangsu 226001, China. 4 Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, School of Basic Medicine, Ningxia Medical University, Yinchuan, 750004, China. Item in Clipboard CiteDisplay options Display options Format Abstract Seminal plasma contains a high concentration of extracellular vesicles (EVs). The heterogeneity of small EVs or the presence of non-vesicular extracellular matter (NV) pose major obstacles in understanding the composition and function of seminal EVs. In this study, we employed high-resolution density gradient fractionation to accurately characterize the composition and function of seminal EVs and NV. We found that the seminal EVs could be divided into three different subtypes, namely high-density EV (EV-H), medium-density EV (EV-M), and low-density EV (EV-L) after purification using iodixanol,while NV was successfully isolated. EVs and NV display different features in size, shape and expression of some classic exosome markers. Both EV-H and NV could markedly promote sperm motility and capacitation compared with EV-M and EV-L, whereas only the NV fraction induced sperm acrosome reaction. Proteomic analysis results showed that EV-H, EV-M, EV-L, and NV had different protein components and were involved in different physiological functions. Further study showed that EV-M might reduce the production of sperm intrinsic reactive oxygen species (ROS) through Glutathione S-transferase Mu 2 (GSTM2).This study provides novel insights into important aspects of seminal EVs constituents and sounder footing to explore their functional properties in male fertility. Keywords: Seminal extracellular vesicles; acrosome reaction; high-resolution iodixanol gradients; proteomics; reactive oxygen species; sperm capacitation. © The Author(s) 2021. Published by Oxford University Press on behalf of the Endocrine Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com. [x] Cite Copy Format: Send To [x]

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