Nanoarrays of Individual Liposomes and Bacterial Outer Membrane Vesicles by Liftoff Nanocontact Printing

Posted: 2021-10-16 19:00:00
Analytical characterization of small biological particles, such as extracellular vesicles (EVs), is complicated by their extreme heterogeneity in size, lipid, membrane protein, and cargo composition. Analysis of individual particles is essential for illuminating particle property distributions that are obscured by ensemble measurements. To enable high-throughput analysis of individual particles, liftoff nanocontact printing (LNCP) is used to define hexagonal antibody and toxin arrays that have a 425 nm dot size, on average, and 700 nm periodicity. The LNCP process is rapid, simple, and does not require access to specialized nanofabrication tools. These densely packed, highly ordered arrays are used to capture liposomes and bacterial outer membrane vesicles on the basis of their surface biomarkers, with a maximum of one particle per array dot, resulting in densely packed arrays of particles. Despite the high particle density, the underlying antibody or toxin array ensured that neighboring individual particles are optically resolvable. Provided target particle biomarkers and suitable capture molecules are identified, this approach can be used to generate high density arrays of a wide variety of small biological particles, including other types of EVs like exosomes. Keywords: aggregatibacter actinomycetemcomitans; extracellular vesicles; liposomes; nanoarrays; nanocontact printing; outer membrane vesicles.

参考サイト PubMed: exsome


10月 16, 2019 バイオアソシエイツ

南カリフォルニアのHOAG 病院は、癌の遺伝的リスクが高い人の癌検出と管理の早期疾患マーカーとしてエクソソームを研究。

カリフォルニア州オレンジ郡にある非営利の地域医療提供ネットワークのHoag Memorial Hospital Presbyterianは、癌診断、癌の進行、および治療抵抗性の初期疾患マーカーの可能性を特定および特徴付ける研究の開始を発表した。 Exosome Sciences社および、Aethlon Medical社の子会社との提携により、癌の遺伝的リスクが高い癌患者の エクソソーム 研究を開始する。…

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