Multi-miRNAs panel of tumor-derived extracellular vesicles as promising diagnostic biomarkers of early-stage breast cancer


Posted: 2021-10-09 19:00:00
Extracellular vesicles (EVs) have been emerging as potential biomarkers for disease monitoring. Especially, tumor-derived EVs (TDEs) are known to carry oncogenic microRNAs (miRNAs), so they can be used for diagnosis of early cancer by analyzing the expression levels of EV-miRNAs circulating in the blood. Here, we rapidly and selectively isolate cancerous EVs expressing breast cancer-derived surface markers CD49f and EpCAM by using our novel microfluidic device within two minutes. Based on seven candidates of miRNAs nominated from The Cancer Genome Atlas (TCGA) database, the expression levels of miRNAs in TDEs were validated in a total of 82 individuals including 62 breast cancer patients and 20 healthy controls. Among seven candidate miRNAs, four miRNAs (miR-9, miR-16, miR-21, and miR-429) from the EVs were highly elevated in early-stage breast cancer patients compared with healthy donors. The combination of significant miRNAs from specific EVs has high sensitivities of 0.90, 0.86, 0.88, and 0.84 of AUC in each subtype (luminal A, luminal B, HER-2, and triple-negative) of early-stage breast cancer. Our results suggest that the combination of four miRNA signatures of specific EVs could serve as a sensitive and specific biomarker enabling early diagnosis of breast cancer using liquid biopsy. Keywords: biomarker; early-stage breast cancer; extracellular vesicles; liquid biopsy; microRNA.

参考サイト PubMed: exsome


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バイオクイックニュース日本語版:エクソソーム特集

バイオクイックニュース日本語版
9月 18, 2019 バイオアソシエイツ

創傷治療治験中のペプチド変異体が、心臓発作の『バイスタンダー効果』による損傷を防御する可能性。研究グループはエクソソームによる防御ペプチドの送達を検討中。

健康な心筋組織を保護することで損傷を減らす心臓発作の直後に服用できる薬があると想像して欲しい。 心臓発作が起きた場合、心臓の専門医は、「時は筋肉なり」と言うと、バージニア工科大学カリリオン心臓医療センター・フラリン生物医学研究所のディレクターであるRobert Gourdie博士(写真)は語った。 血流によって酸素が供給されないと、心臓細胞はすぐに死ぬ。…

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