Method for Isolating Extracellular Vesicles from Human Neural Stem Cells Expanded Under Neurosphere Culture


Posted: 2021-09-24 19:00:00
Methods Mol Biol . 2022;2389:87-94. doi: 10.1007/978-1-0716-1783-0_8. Affiliations Expand Affiliations 1 Department of Pharmaceutics, College of Pharmacy, The University of Florida, Gainesville, FL, USA. 2 Department of Neurosurgery, College of Medicine, The University of Florida, Gainesville, FL, USA. 3 Department of Neurosurgery, College of Medicine, The University of Florida, Gainesville, FL, USA. hassan.azari@neurosurgery.ufl.edu. Item in Clipboard Nasser Nassiri Koopaei et al. Methods Mol Biol. 2022. Show details Display options Display options Format Methods Mol Biol . 2022;2389:87-94. doi: 10.1007/978-1-0716-1783-0_8. Affiliations 1 Department of Pharmaceutics, College of Pharmacy, The University of Florida, Gainesville, FL, USA. 2 Department of Neurosurgery, College of Medicine, The University of Florida, Gainesville, FL, USA. 3 Department of Neurosurgery, College of Medicine, The University of Florida, Gainesville, FL, USA. hassan.azari@neurosurgery.ufl.edu. Item in Clipboard CiteDisplay options Display options Format Abstract Neural stem cells (NSCs) transplantation enhances plasticity and restores functions in neurological diseases. Therapeutic benefits of NSCs are due to their ability to replace the lost neurons and glial cells and also secreting a wide array of free and membrane-bound bioactive molecules that can reduce the hostility of diseased microenvironment, resolve inflammation, and rescue damaged neural cells. Membrane-encircled spherical nanostructures that are collectively known as extracellular vesicles (EVs) contain mRNA, miRNA, lipids, and specific proteins that affect different biological processes in cells located nearby or at far distances. Using EVs as an alternative non-cell-based therapy has gained huge attention, and developing methods for large-scale production of EVs is of great clinical importance. Here, we describe an efficient method to yield significant quantity of EVs from human NSCs that are expanded under free floating neurosphere assay culture system. Using the neurosphere assay in bioreactors under GMP-compliant conditions can result in scalable NSC-EVs required for human trials. Keywords: Extracellular vesicles; Neural stem cells; Neurosphere assay; Production and isolation; Regenerative medicine. © 2022. Springer Science+Business Media, LLC, part of Springer Nature. References Colombo M, Raposo G, Thery C (2014) Biogenesis, secretion, and intercellular interactions of exosomes and other extracellular vesicles. Annu Rev Cell Dev Biol 30:255–289 - DOI Sutaria DS et al (2017) Achieving the promise of therapeutic extracellular vesicles: the devil is in details of therapeutic loading. Pharm Res 34(5):1053–1066 - DOI Colao IL et al (2018) Manufacturing exosomes: a promising therapeutic platform. Trends Mol Med 24(3):242–256 - DOI Valadi H et al (2007) Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nat Cell Biol 9(6):654–659 - DOI Branscome H et al (2020) Use of stem cell extracellular vesicles as a “holistic” approach to CNS repair. Front Cell Dev Biol 8:455 - DOI Show all 18 references [x] Cite Copy Format: Send To [x]

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バイオクイックニュース日本語版:エクソソーム特集

バイオクイックニュース日本語版
1月 22, 2019 バイオアソシエイツ

化学療法は乳癌細胞から転移促進エキソソームの放出を刺激し、エクソソームはその内容物を肺に放出することがある。

乳癌患者の中には、腫瘍が手術で取り除かれる前に化学療法を受ける人もいる。 ネオアジュバント療法と呼ばれるこのアプローチは、乳房温存手術を容易にするために腫瘍のサイズを縮小するのを助け、外科医が除去するための癌性細胞をほとんどまたは全く残さずに腫瘍を根絶することさえできる。 そのような場合、患者は手術後の生涯に渡り癌のないまま過ごせる可能性が高い。 しかし、すべての腫瘍が化学療法で縮小するわけではない。…

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