Mesenchymal Stem Cell-Derived Exosomes Attenuate TLR7-Mediated Mast Cell Activation


Posted: 2021-11-18 20:00:00
Background: Mast cells are immune sentinels in the skin that respond to a wide range of pathological and environmental stimuli; they owe their function to the expression of Toll-like receptors (TLRs). We previously found that tonsil-derived mesenchymal stem cells (T-MSCs) were able to effectively attenuate TLR7-mediated skin inflammation in mice, which was accompanied by an increase in mast cell number. The present study investigated whether T-MSC extracellular vesicles, such as exosomes, are able to regulate mast cell activation in response to TLR7 stimulation. Methods: The HMC-1 human mast cell line was treated with a TLR7 agonist in the presence or absence of T-MSC exosomes, and the levels of expressed inflammatory cytokines were assessed. Additionally, mice were repeatedly injected with a TLR7 agonist with or without interval treatments with T-MSC exosomes and assessed dermal distribution of mast cells and related immune cells. Results: We showed that T-MSC exosomes containing microRNAs that target inflammatory cytokines significantly reduced the expression of inflammatory cytokines in TLR7 agonist-treated HMC-1 cells. In addition, T-MSC exosomes inhibited the increase in the number of both dermal mast cells and CD14-positive cells in TLR7 agonist-treated mice. Conclusion: Our data suggest that T-MSC exosomes have regulatory effects on mast cell activation under inflammatory conditions, including TLR7 stimulation. Keywords: Exosomes; Mast cell; Mesenchymal stem cell; TLR7.

参考サイト PubMed: exsome



バイオクイックニュース日本語版:エクソソーム特集

バイオクイックニュース日本語版
11月 16, 2021 バイオアソシエイツ

癌免疫療法を支援するために、免疫反応と腫瘍微小環境を共同で活性化するキメラ型エクソソームが開発された

中国科学院プロセス工学研究所(IPE)の研究者らは、癌免疫療法を支援するために、免疫反応と腫瘍微小環境を共同で活性化するマクロファージと腫瘍のキメラ型エクソソームを開発した。この研究は、Science Translational Medicine誌のオンライン版に2021年10月13日に掲載された。この論文は、「マクロファージと腫瘍のキメラ型エクソソームがリンパ節と腫瘍に蓄積し、免疫反応と腫瘍微小環境を活性化する(Macrophage-Tumor Chimeric Exosomes Accumulate in…

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