Membrane Vesicles Produced by Shewanella vesiculosa HM13 as a Prospective Platform for Secretory Production of Heterologous Proteins at Low Temperatures


Posted: 2021-11-16 20:00:00
Methods Mol Biol . 2022;2414:191-205. doi: 10.1007/978-1-0716-1900-1_12. Affiliations Expand Affiliations 1 Institute for Chemical Research, Kyoto University, Uji, Kyoto, Japan. 2 Institute for Chemical Research, Kyoto University, Uji, Kyoto, Japan. kurihara@scl.kyoto-u.ac.jp. Item in Clipboard Jun Kawamoto et al. Methods Mol Biol. 2022. Show details Display options Display options Format Methods Mol Biol . 2022;2414:191-205. doi: 10.1007/978-1-0716-1900-1_12. Affiliations 1 Institute for Chemical Research, Kyoto University, Uji, Kyoto, Japan. 2 Institute for Chemical Research, Kyoto University, Uji, Kyoto, Japan. kurihara@scl.kyoto-u.ac.jp. Item in Clipboard CiteDisplay options Display options Format Abstract Extracellular membrane vesicles (EMVs) produced by Gram-negative bacteria are useful as a vaccine platform. During growth in broth at 18 °C, Shewanella vesiculosa HM13 produces a large number of EMVs that contain a 49-kDa major cargo protein, named P49. Enhanced green fluorescent protein fused to the C-terminus of P49 is delivered to EMVs, suggesting that P49 is useful as a carrier to target foreign proteins to EMVs for production of artificial EMVs with desired functions. This method is potentially useful for the preparation of designed vaccines and is described in detail in this chapter. Keywords: Extracellular membrane vesicles; Protein fusion; Protein secretion; Protein transport; Recombinant protein production. © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. References Schwechheimer C, Kuehn MJ (2015) Outer-membrane vesicles from Gram-negative bacteria: biogenesis and functions. Nat Rev Microbiol 13:605–619. https://doi.org/10.1038/nrmicro3525 Toyofuku M, Tashiro Y, Hasegawa Y et al (2015) Bacterial membrane vesicles, an overlooked environmental colloid: biology, environmental perspectives and applications. Adv Colloid Interf Sci 226:65–77. https://doi.org/10.1016/j.cis.2015.08.013 - DOI Nagakubo T, Nomura N, Toyofuku M (2020) Cracking open bacterial membrane vesicles. Front Microbiol 10:3026. https://doi.org/10.3389/fmicb.2019.03026 - DOI - PubMed - PMC Anand D, Chaudhuri A (2016) Bacterial outer membrane vesicles: new insights and applications. Mol Membr Biol 33:125–137. https://doi.org/10.1080/09687688.2017.1400602 - DOI - PubMed McCarthy PC, Sharyan A, Sheikhi Moghaddam L (2018) Meningococcal vaccines: current status and emerging strategies. Vaccines (Basel) 6:12. https://doi.org/10.3390/vaccines6010012 - DOI Show all 18 references

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バイオクイックニュース日本語版:エクソソーム特集

バイオクイックニュース日本語版
10月 16, 2019 バイオアソシエイツ

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