In vivo imaging of EVs in zebrafish: New perspectives from "the waterside"


Posted: 2021-11-11 20:00:00
Review FASEB Bioadv . 2021 Aug 27;3(11):918-929. doi: 10.1096/fba.2021-00081. eCollection 2021 Nov. Affiliations Expand Affiliations 1 INSERM U1266 Institut de Psychiatrie et Neurosciences de Paris Paris France. 2 Groupe Hospitalier Universitaire (GHU) Paris Paris France. Item in Clipboard Review Vincenzo Verdi et al. FASEB Bioadv. 2021. Show details Display options Display options Format FASEB Bioadv . 2021 Aug 27;3(11):918-929. doi: 10.1096/fba.2021-00081. eCollection 2021 Nov. Affiliations 1 INSERM U1266 Institut de Psychiatrie et Neurosciences de Paris Paris France. 2 Groupe Hospitalier Universitaire (GHU) Paris Paris France. Item in Clipboard CiteDisplay options Display options Format Abstract To harmoniously coordinate the activities of all its different cell types, a multicellular organism critically depends on intercellular communication. One recently discovered mode of intercellular cross-talk is based on the exchange of "extracellular vesicles" (EVs). EVs are nano-sized heterogeneous lipid bilayer vesicles enriched in a variety of biomolecules that mediate short- and long-distance communication between different cells, and between cells and their environment. Numerous studies have demonstrated important aspects pertaining to the dynamics of their release, their uptake, and sub-cellular fate and roles in vitro. However, to demonstrate these and other aspects of EV biology in a relevant, fully physiological context in vivo remains challenging. In this review we analyze the state of the art of EV imaging in vivo, focusing in particular on zebrafish as a promising model to visualize, study, and characterize endogenous EVs in real-time and expand our understanding of EV biology at cellular and systems level. Keywords: exosomes; extracellular vesicles; homeostasis; live‐imaging; zebrafish. © 2021 The Authors. FASEB BioAdvances published by The Federation of American Societies for Experimental Biology. Conflict of interest statement Figures FIGURE 1 EV sub‐populations released by a… FIGURE 1 EV sub‐populations released by a single cell, with their respective diameters. EV, extracellular… FIGURE 1 EV sub‐populations released by a single cell, with their respective diameters. EV, extracellular vesicles; MVB, multivesicular body; N, nucleus FIGURE 2 Zebrafish (ZF) embryos as comprehensive… FIGURE 2 Zebrafish (ZF) embryos as comprehensive model to investigate EV‐biology in vivo. Live‐imaging of… FIGURE 2 Zebrafish (ZF) embryos as comprehensive model to investigate EV‐biology in vivo. Live‐imaging of genetically labelled endogenous EVs in the ZF embryo allows to (1) study their release by producing cells, (2) follow their journey in the bloodstream and interstitial compartments, (3) follow their uptake by their natural targets and (4) characterize their intracellular fate. EV, extracellular vesicles FIGURE 3 Visualization and mapping of the… FIGURE 3 Visualization and mapping of the endogenous “inter organ EV‐interactome” in the ZF embryo.… FIGURE 3 Visualization and mapping of the endogenous “inter organ EV‐interactome” in the ZF embryo. Tissue‐specific expression of EV (‐subpopulation) reporter‐proteins as well as cargo‐transfer reporter systems could help unravel EV‐mediated communication pathways existing between different tissues and organs. EV, extracellular vesicles; ZF, zebrafish FIGURE 4 Options to interfere with endogenous… FIGURE 4 Options to interfere with endogenous EV biology in vivo. To better understand and… FIGURE 4 Options to interfere with endogenous EV biology in vivo. To better understand and pinpoint the (patho) physiological roles of endogenous EVs in vivo, various developments are necessary. (Upper half) Hypothetical model of the various steps during the normal life‐span of endogenous EVs in vivo. (Lower half) Opportunities to interfere. (1) Spatial‐ and/or temporal modulation of EV secretion. (2) Modulation of the natural “default” EVs trajectory toward a different, ectopic target. (3) Genetic control of endocytosis and of the intracellular fate in recipient cells. EV, extracellular vesicles References Wolf P. The nature and significance of platelet products in human plasma. Br J Haematol. 1967;13:269‐288. - PubMed Cruz L, Romero JAA, Iglesia RP, Lopes MH. Extracellular vesicles: decoding a new language for cellular communication in early embryonic development. Front Cell Dev Biol. 2018;6:1‐12. - PMC - PubMed Lopera‐Vásquez R, Hamdi M, Fernandez‐Fuertes B, et al. Extracellular vesicles from BOEC in in vitro embryo development and quality. PLoS One. 2016;11:e0148083. - PMC - PubMed Stahl PD, Raposo G. Extracellular vesicles: exosomes and microvesicles. Integrators of homeostasis. Physiology. 2019;34:169‐177. - PubMed Eitan E, Hutchison ER, Marosi K, et al. Extracellular vesicle‐associated Aβ mediates trans‐neuronal bioenergetic and Ca2+‐handling deficits in Alzheimer’s disease models. NPJ Aging Mech Dis. 2016;2:16019. - PMC - PubMed Show all 115 references Publication types LinkOut - more resources Research Materials [x] Cite Copy Format: Send To [x]

参考サイト PubMed: exsome



バイオクイックニュース日本語版:エクソソーム特集

バイオクイックニュース日本語版
12月 17, 2019 バイオアソシエイツ

エクソソームが重度の前立腺癌促進伝達因子の送達をしていることが判明。 エクソソーム放出阻害が治療に有用であることが証明された。

ニューロン機能を支援する転写因子は、すでに再発した癌をさらに致命的にする可能性のある前立腺の細胞変換を可能にするようだ。 転写因子BRN4は主に中枢神経系と内耳で発現するが、稀であるが神経内分泌前立腺癌の患者でも増幅され過剰発現する最初の証拠がClinical Cancer Researchジャーナルで公開された。 この論文は「BRN4は去勢抵抗性前立腺癌における神経内分泌分化の新規ドライバーであり、BRN2を含む細胞外小胞で選択的に放出される(BRN4 Is a Novel Driver of…

ゲスト 521人 と メンバー 6人 がオンラインです