Giant plasma membrane vesicles to study plasma membrane structure and dynamics

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Posted: 2022-01-06 20:00:00
Biochim Biophys Acta Biomembr . 2022 Jan 3;183857. doi: 10.1016/j.bbamem.2021.183857. Online ahead of print. Affiliations Expand Affiliation 1 Science for Life Laboratory, Department of Women's and Children's Health, Karolinska Institutet, 17165 Solna, Sweden. Electronic address: erdinc.sezgin@ki.se. Item in Clipboard Erdinc Sezgin. Biochim Biophys Acta Biomembr. 2022. Show details Display options Display options Format Biochim Biophys Acta Biomembr . 2022 Jan 3;183857. doi: 10.1016/j.bbamem.2021.183857. Online ahead of print. Affiliation 1 Science for Life Laboratory, Department of Women's and Children's Health, Karolinska Institutet, 17165 Solna, Sweden. Electronic address: erdinc.sezgin@ki.se. Item in Clipboard CiteDisplay options Display options Format Abstract The plasma membrane (PM) is a highly heterogenous structure intertwined with the cortical actin cytoskeleton and extracellular matrix. This complex architecture makes it difficult to study the processes taking place at the PM. Model membrane systems that are simple mimics of the PM overcome this bottleneck and allow us to study the biophysical principles underlying the processes at the PM. Among them, cell-derived giant plasma membrane vesicles (GPMVs) are considered the most physiologically relevant system, retaining the compositional complexity of the PM to a large extent. GPMVs have become a key tool in membrane research in the last few years. In this review, I will provide a brief overview of this system, summarize recent applications and discuss the limitations. Keywords: Drug delivery; Giant plasma membrane vesicles; Lipid-protein interactions; Lipidomics; Membrane domains; Model membranes; Phase separation; Plasma membrane. Copyright © 2021. Published by Elsevier B.V. Conflict of interest statement Declaration of competing interest The author declares no conflict of interest. Similar articles Cell-Derived Plasma Membrane Vesicles Are Permeable to Hydrophilic Macromolecules. Skinkle AD, Levental KR, Levental I. Skinkle AD, et al. Biophys J. 2020 Mar 24;118(6):1292-1300. doi: 10.1016/j.bpj.2019.12.040. Epub 2020 Jan 28. Biophys J. 2020. PMID: 32053777 Free PMC article. Giant Endoplasmic Reticulum vesicles (GERVs), a novel model membrane tool. Grimmer M, Bacia K. Grimmer M, et al. Sci Rep. 2020 Feb 20;10(1):3100. doi: 10.1038/s41598-020-59700-1. Sci Rep. 2020. PMID: 32080222 Free PMC article. Diffusion of Single-Pass Transmembrane Receptors: From the Plasma Membrane into Giant Liposomes. Worch R, Petrášek Z, Schwille P, Weidemann T. Worch R, et al. J Membr Biol. 2017 Aug;250(4):393-406. doi: 10.1007/s00232-016-9936-8. Epub 2016 Nov 8. J Membr Biol. 2017. PMID: 27826635 Free PMC article. Giant plasma membrane vesicles: models for understanding membrane organization. Levental KR, Levental I. Levental KR, et al. Curr Top Membr. 2015;75:25-57. doi: 10.1016/bs.ctm.2015.03.009. Epub 2015 Apr 17. Curr Top Membr. 2015. PMID: 26015280 Review. Model membrane platforms to study protein-membrane interactions. Sezgin E, Schwille P. Sezgin E, et al. Mol Membr Biol. 2012 Aug;29(5):144-54. doi: 10.3109/09687688.2012.700490. Epub 2012 Jul 26. Mol Membr Biol. 2012. PMID: 22831167 Review. [x] Cite Copy Format: Send To [x]

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