Extruded Mesenchymal Stem Cell Nanovesicles Are Equally Potent to Natural Extracellular Vesicles in Cardiac Repair


Posted: 2021-11-18 20:00:00
ACS Appl Mater Interfaces . 2021 Nov 18. doi: 10.1021/acsami.1c08044. Online ahead of print. Affiliations Expand Affiliations 1 Cell Therapy Laboratory, The First Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, China. 2 Department of Cardiology, The First Hospital of Hebei Medical University, Shijiazhuang 050000, China. 3 Hebei Key Laboratory of Heart and Metabolism, The First Hospital of Hebei Medical University, Shijiazhuang 050000, China. 4 Department of Molecular Biomedical Science, North Carolina State University, Raleigh, 27607 North Carolina, United States. 5 Department of Biomedical Engineering, University of North Carolina, Chapel Hill and North Carolina State University, Raleigh, 27607 North Carolina, United States. Item in Clipboard Xianyun Wang et al. ACS Appl Mater Interfaces. 2021. Show details Display options Display options Format ACS Appl Mater Interfaces . 2021 Nov 18. doi: 10.1021/acsami.1c08044. Online ahead of print. Affiliations 1 Cell Therapy Laboratory, The First Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, China. 2 Department of Cardiology, The First Hospital of Hebei Medical University, Shijiazhuang 050000, China. 3 Hebei Key Laboratory of Heart and Metabolism, The First Hospital of Hebei Medical University, Shijiazhuang 050000, China. 4 Department of Molecular Biomedical Science, North Carolina State University, Raleigh, 27607 North Carolina, United States. 5 Department of Biomedical Engineering, University of North Carolina, Chapel Hill and North Carolina State University, Raleigh, 27607 North Carolina, United States. Item in Clipboard Full text links CiteDisplay options Display options Format Abstract Mesenchymal stem cells (MSCs) repair injured tissues mainly through their paracrine actions. One of the important paracrine components of MSC secretomes is the extracellular vesicle (EV). The therapeutic potential of MSC-EVs has been established in various cardiac injury preclinical models. However, the large-scale production of EVs remains a challenge. We sought to develop a scale-up friendly method to generate a large number of therapeutic nanovesicles from MSCs by extrusion. Those extruded nanovesicles (NVs) are miniature versions of MSCs in terms of surface marker expression. The yield of NVs is 20-fold more than that of EVs. In vitro, cell-based assays demonstrated the myocardial protective effects and therapeutic potential of NVs. Intramyocardial delivery of NVs in the injured heart after ischemia-reperfusion led to a reduction in scar sizes and preservation of cardiac functions. Such therapeutic benefits are similar to those injected with natural EVs from the same MSC parental cells. In addition, NV therapy promoted angiogenesis and proliferation of cardiomyocytes in the post-injury heart. In summary, extrusion is a highly efficient method to generate a large quantity of therapeutic NVs that can potentially replace extracellular vesicles in regenerative medicine applications. Keywords: extracellular vesicles; extrusion; ischemia reperfusion injury; mesenchymal stem cell; nanovesicles. LinkOut - more resources Full Text Sources

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