Exosomes derived from PC-3 cells suppress osteoclast differentiation by downregulating miR-148a and blocking the PI3K/AKT/mTOR pathway


Posted: 2021-10-11 19:00:00
Exp Ther Med . 2021 Nov;22(5):1304. doi: 10.3892/etm.2021.10739. Epub 2021 Sep 16. Affiliations Expand Affiliations 1 Department of Spine Surgery, The Affiliated Yuebei People's Hospital of Shantou University Medical College, Shaoguan, Guangdong 512025, P.R. China. 2 Department of Orthopedics, Zhujiang Hospital, The Second School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong 510280, P.R. China. 3 Department of Oral and Maxillofacial Surgery, Stomatological Hospital of Southern Medical University, Guangzhou, Guangdong 510250, P.R. China. Item in Clipboard Gaoqiang Tian et al. Exp Ther Med. 2021 Nov. Show details Display options Display options Format Exp Ther Med . 2021 Nov;22(5):1304. doi: 10.3892/etm.2021.10739. Epub 2021 Sep 16. Affiliations 1 Department of Spine Surgery, The Affiliated Yuebei People's Hospital of Shantou University Medical College, Shaoguan, Guangdong 512025, P.R. China. 2 Department of Orthopedics, Zhujiang Hospital, The Second School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong 510280, P.R. China. 3 Department of Oral and Maxillofacial Surgery, Stomatological Hospital of Southern Medical University, Guangzhou, Guangdong 510250, P.R. China. Item in Clipboard CiteDisplay options Display options Format Abstract Prostate cancer is a leading malignancy in men that can also disrupt the bone tissue balance. Among all urological cancers, prostate cancer is associated with the highest rate of bone metastases, which can greatly reduce a patient's quality of life. In recent years, cell-derived exosomes, which can contain a wide range of biologically active molecules, have been reported as a novel method of communication among individual cells. However, the specific role that exosomes serve in this disease has not been fully elucidated. The prostate cancer cell line PC-3 were applied in the present study, where its exosomes were isolated to explore their potential effects on osteoclast differentiation. Exosomes are extracellular vesicles secreted by cells. The size of exosomes is 30-150 nm. They have double membrane structure and saucer-like morphology. They contain rich contents (including nucleic acid, protein and lipid) and participate in molecular transmission between cells. The combined results of tartrate-resistant acid phosphatase staining (to identify osteoclasts obtained from human peripheral blood mononuclear cells), reverse transcription-quantitative PCR and western blotting showed that PC-3-derived exosomes attenuated osteoclast differentiation by downregulating marker genes associated with osteoclastic maturation, including V-maf musculoaponeurotic fibrosarcoma oncogene homolog B, matrix metalloproteinase 9 and integrin β3. microRNA (miR)-148a expression was also found to be downregulated in osteoclasts by PC-3-derived exosomes. In addition, the mTOR and AKT signaling pathways were blocked after exposure to these PC-3 cell-derived exosomes. Therefore, results from the present study suggest that miR-148a mimics may be a new therapeutic approach for the prevention of prostate cancer bone metastases. Keywords: PC-3 cells; exosome; microRNA-148a; osteoclast; prostate cancer. Copyright: © Tian et al. Conflict of interest statement The authors declare they have no competing interests. [x] Cite Copy Format: Send To [x]

参考サイト PubMed: exsome



バイオクイックニュース日本語版:エクソソーム特集

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2月 19, 2021 バイオアソシエイツ

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ミシガン大学ローゲル癌センターとミシガン大学工学部の研究者らは、癌と戦うためのナチュラルキラー免疫細胞の配備という新たな治療法の開発の面で一歩進んでいる。この研究者らは、ナチュラルキラー細胞をキャプチャーし、それらに癌を殺す エクソソーム を放出させる最初の体系的な方法を開発した。…

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