Dendrobium officinale Polysaccharide Alleviates Intestinal Inflammation by Promoting Small Extracellular Vesicle Packaging of miR-433-3p


Posted: 2021-11-05 19:00:00
J Agric Food Chem . 2021 Nov 5. doi: 10.1021/acs.jafc.1c05134. Online ahead of print. Huifan Liu 1 2 , Jiaxi Liang 1 2 , Yuming Zhong 3 , Gengsheng Xiao 1 2 , Thomas Efferth 4 , Milen I Georgiev 5 6 , Celia Vargas-De-La-Cruz 7 8 , Vivek K Bajpai 9 , Giovanni Caprioli 10 , Jianliang Liu 11 , Jintian Lin 12 , Hui Wu 13 , Lianxin Peng 14 , Yongjun Li 15 , Lukai Ma 1 2 , Jianbo Xiao 16 , Qin Wang 1 2 Affiliations Expand Affiliations 1 Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology, Guangzhou City, Guangdong 510642, China. 2 College of Light Industry and Food, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, China. 3 College of Resources and Environment, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, China. 4 Department of Pharmaceutical Biology, Johannes Gutenberg University, 55128 Mainz, Germany. 5 University of Agronomic Science and Veterinary Medicine, 59 Marasti Blvd, 011464 Bucharest, Romania. 6 Laboratory of Metabolomics, Institute of Microbiology, Bulgarian Academy of Sciences, 139 Ruski Boulevard, 4000 Plovdiv, Bulgaria. 7 Faculty of Pharmacy and Biochemistry, Academic Department of Pharmacology, Bromatology and Toxicology, Centro Latinoamericano de Enseñanza e Investigación en Bacteriología Alimentaria (CLEIBA), Universidad Nacional Mayor de San Marcos, Lima 15001, Perú. 8 Research Group Biotechnology and Omics in Life Sciences, Universidad Nacional Mayor de San Marcos, Lima 15001, Perú. 9 Department of Energy and Materials Engineering, Dongguk University, 30 Pildong-ro 1-gil, Seoul 04620, Republic of Korea. 10 School of Pharmacy, University of Camerino, Via Sant'Agostino 1, 62032 Camerino, MC, Italy. 11 Modern Agriculture Research Center, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, China. 12 College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, China. 13 School of Food Science and Engineering, South China University of Technology, Guangzhou 510006, Guangdong, China. 14 Key Laboratory of Coarse Cereal Processing of Ministry of Agriculture and Rural Affairs, Chengdu University, Chengdu, Sichuan 610106, China. 15 Guangdong Jiangmen Chinese Medical College, Jiangmen City 529000, Guangdong China. 16 Nutrition and Bromatology Group, Analytical and Food Chemistry Department, Faculty of Food Science and Technology, University of Vigo, Ourense Campus, E-32004 Ourense, Spain. Item in Clipboard Huifan Liu et al. J Agric Food Chem. 2021. Show details Display options Display options Format J Agric Food Chem . 2021 Nov 5. doi: 10.1021/acs.jafc.1c05134. Online ahead of print. Authors Huifan Liu 1 2 , Jiaxi Liang 1 2 , Yuming Zhong 3 , Gengsheng Xiao 1 2 , Thomas Efferth 4 , Milen I Georgiev 5 6 , Celia Vargas-De-La-Cruz 7 8 , Vivek K Bajpai 9 , Giovanni Caprioli 10 , Jianliang Liu 11 , Jintian Lin 12 , Hui Wu 13 , Lianxin Peng 14 , Yongjun Li 15 , Lukai Ma 1 2 , Jianbo Xiao 16 , Qin Wang 1 2 Affiliations 1 Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology, Guangzhou City, Guangdong 510642, China. 2 College of Light Industry and Food, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, China. 3 College of Resources and Environment, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, China. 4 Department of Pharmaceutical Biology, Johannes Gutenberg University, 55128 Mainz, Germany. 5 University of Agronomic Science and Veterinary Medicine, 59 Marasti Blvd, 011464 Bucharest, Romania. 6 Laboratory of Metabolomics, Institute of Microbiology, Bulgarian Academy of Sciences, 139 Ruski Boulevard, 4000 Plovdiv, Bulgaria. 7 Faculty of Pharmacy and Biochemistry, Academic Department of Pharmacology, Bromatology and Toxicology, Centro Latinoamericano de Enseñanza e Investigación en Bacteriología Alimentaria (CLEIBA), Universidad Nacional Mayor de San Marcos, Lima 15001, Perú. 8 Research Group Biotechnology and Omics in Life Sciences, Universidad Nacional Mayor de San Marcos, Lima 15001, Perú. 9 Department of Energy and Materials Engineering, Dongguk University, 30 Pildong-ro 1-gil, Seoul 04620, Republic of Korea. 10 School of Pharmacy, University of Camerino, Via Sant'Agostino 1, 62032 Camerino, MC, Italy. 11 Modern Agriculture Research Center, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, China. 12 College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, China. 13 School of Food Science and Engineering, South China University of Technology, Guangzhou 510006, Guangdong, China. 14 Key Laboratory of Coarse Cereal Processing of Ministry of Agriculture and Rural Affairs, Chengdu University, Chengdu, Sichuan 610106, China. 15 Guangdong Jiangmen Chinese Medical College, Jiangmen City 529000, Guangdong China. 16 Nutrition and Bromatology Group, Analytical and Food Chemistry Department, Faculty of Food Science and Technology, University of Vigo, Ourense Campus, E-32004 Ourense, Spain. Item in Clipboard CiteDisplay options Display options Format Abstract Dendrobium officinale polysaccharide (DOP) attenuates inflammatory bowel disease (IBD), but its role in regulating cross-talk between intestinal epithelial cells (IEC) and macrophages against IBD is unclear. This study aimed to investigate DOP protective effects on the intestinal inflammatory response through regulation by miRNA in small extracellular vesicles (sEVs). Our results show that DOP interfered with the secretion of small extracellular vesicles (DIEs) by IEC, which reduced the levels of inflammatory mediators. Increased miR-433-3p expression in DIEs was identified as an important protector against intestinal inflammation. DOP regulated the loading of miR-433-3p by hnRNPA2B1 into the intestinal sEV to increase the abundance of miR-433-3p. DIEs delivered miR-433-3p to lipopolysaccharide-induced macrophages and targeted the MAPK8 gene, leading to inhibition of the MAPK signaling pathway and reduced production of inflammatory cytokines. One protective mechanism of DOP is mediated by intestinal sEV containing miR-433-3p, which is a potential therapeutic agent for the prevention of inflammatory factor accumulation from excessive intestinal macrophage activity and for restoring homeostasis in the intestinal microenvironment. Keywords: Dendrobium officinale polysaccharide; inflammatory bowel disease; intestinal small extracellular vesicles; miR-433-3p.

参考サイト PubMed: exsome



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