Contributing to Understand the Crosstalk between Brain and Periphery in Methylmercury Intoxication: Neurotoxicity and Extracellular Vesicles


Posted: 2021-10-13 19:00:00
Int J Mol Sci . 2021 Oct 7;22(19):10855. doi: 10.3390/ijms221910855. Affiliations Expand Affiliations 1 Laboratório de Farmacologia Molecular, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém 66075-110, Brazil. 2 Department of Pharmacology, University of Oxford, Oxford OX1 3QT, UK. 3 Laboratory of Pharmacology of Inflammation and Behavior, Faculty of Pharmacy, Institute of Health Science, Federal University of Pará, Belém 66075-110, Brazil. Item in Clipboard Gabriela de Paula Arrifano et al. Int J Mol Sci. 2021. Show details Display options Display options Format Int J Mol Sci . 2021 Oct 7;22(19):10855. doi: 10.3390/ijms221910855. Affiliations 1 Laboratório de Farmacologia Molecular, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém 66075-110, Brazil. 2 Department of Pharmacology, University of Oxford, Oxford OX1 3QT, UK. 3 Laboratory of Pharmacology of Inflammation and Behavior, Faculty of Pharmacy, Institute of Health Science, Federal University of Pará, Belém 66075-110, Brazil. Item in Clipboard CiteDisplay options Display options Format Abstract Human exposure to methylmercury (MeHg) is currently high in regions such as the Amazon. Understanding the molecular changes associated with MeHg-induced neurotoxicity and the crosstalk with the periphery is essential to support early diagnoses. This work aimed to evaluate cellular and molecular changes associated with behavioral alterations in MeHg acute exposure and the possible changes in extracellular vesicles (EVs) number and S100β content. Adults male Wistar rats were orally treated with 5 mg/kg for four days. Behavioral performance, molecular and histological changes in the cerebellum, and plasma EVs were assessed. MeHg-intoxicated animals performed significantly worse in behavioral tests. MeHg increased the number of GFAP+ cells and GFAP and S100β mRNA expression in the cerebellum but no change in NeuN+ or IBA-1+ cells number was detected. The number of exosomes isolated from plasma were decreased by the metal. S100B mRNA was detected in circulating plasma EVs cargo in MeHg exposure. Though preliminary, our results suggest astrocytic reactivity is displaying a protective role once there was no neuronal death. Interestingly, the reduction in exosomes number could be a new mechanism associated with MeHg-induced neurotoxicity and plasma EVs could represent a source of future biomarkers in MeHg intoxication. Keywords: CNS; MeHg; S100b; exosomes; exposure; mercury; pollutant; pollution; qPCR; real time. Grant support

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