Characterization of Total RNA, CD44, FASN, and PTEN mRNAs from Extracellular Vesicles as Biomarkers in Gastric Cancer Patients

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Posted: 2021-12-10 20:00:00
Cancers (Basel) . 2021 Nov 27;13(23):5975. doi: 10.3390/cancers13235975. Philipp Rhode 1 , Matthias Mehdorn 1 , Orestis Lyros 1 , Christoph Kahlert 2 , Thomas Kurth 3 , Tom Venus 4 , Katrin Schierle 5 , Irina Estrela-Lopis 4 , Boris Jansen-Winkeln 1 , Florian Lordick 6 7 , Ines Gockel 1 , René Thieme 1 Affiliations Expand Affiliations 1 Department of Visceral, Transplant, Thoracic and Vascular Surgery, University Hospital Leipzig, D-04103 Leipzig, Germany. 2 Department for Visceral, Thoracic and Vascular Surgery, University Hospital Carl Gustav Carus, D-01307 Dresden, Germany. 3 Center for Molecular and Cellular Bioengineering (CMCB), Technology Platform, Technische Universität Dresden, D-01307 Dresden, Germany. 4 Institute of Medical Physics and Biophysics, University of Leipzig, D-0407 Leipzig, Germany. 5 Institute of Pathology, University Hospital Leipzig, D-04103 Leipzig, Germany. 6 Department of Oncology, Gastroenterology, Hepatology, Pulmonology and Infectious Diseases, University Hospital Leipzig, D-04103 Leipzig, Germany. 7 University Cancer Center Leipzig (UCCL), University Hospital Leipzig, D-04103 Leipzig, Germany. Item in Clipboard Philipp Rhode et al. Cancers (Basel). 2021. Show details Display options Display options Format Cancers (Basel) . 2021 Nov 27;13(23):5975. doi: 10.3390/cancers13235975. Authors Philipp Rhode 1 , Matthias Mehdorn 1 , Orestis Lyros 1 , Christoph Kahlert 2 , Thomas Kurth 3 , Tom Venus 4 , Katrin Schierle 5 , Irina Estrela-Lopis 4 , Boris Jansen-Winkeln 1 , Florian Lordick 6 7 , Ines Gockel 1 , René Thieme 1 Affiliations 1 Department of Visceral, Transplant, Thoracic and Vascular Surgery, University Hospital Leipzig, D-04103 Leipzig, Germany. 2 Department for Visceral, Thoracic and Vascular Surgery, University Hospital Carl Gustav Carus, D-01307 Dresden, Germany. 3 Center for Molecular and Cellular Bioengineering (CMCB), Technology Platform, Technische Universität Dresden, D-01307 Dresden, Germany. 4 Institute of Medical Physics and Biophysics, University of Leipzig, D-0407 Leipzig, Germany. 5 Institute of Pathology, University Hospital Leipzig, D-04103 Leipzig, Germany. 6 Department of Oncology, Gastroenterology, Hepatology, Pulmonology and Infectious Diseases, University Hospital Leipzig, D-04103 Leipzig, Germany. 7 University Cancer Center Leipzig (UCCL), University Hospital Leipzig, D-04103 Leipzig, Germany. Item in Clipboard CiteDisplay options Display options Format Abstract In-depth characterization has introduced new molecular subtypes of gastric cancer (GC). To identify these, new approaches and techniques are required. Liquid biopsies are trendsetting and provide an easy and feasible method to identify and to monitor GC patients. In a prospective cohort of 87 GC patients, extracellular vesicles (EVs) were isolated from 250 µL of plasma. The total RNA was isolated with TRIZOL. The total RNA amount and the relative mRNA levels of CD44, PTEN, and FASN were measured by qRT-PCR. The isolation of EVs and their contained mRNA was possible in all 87 samples investigated. The relative mRNA levels of PTEN were higher in patients already treated by chemotherapy than in chemo-naïve patients. In patients who had undergone neoadjuvant chemotherapy followed by gastrectomy, a decrease in the total RNA amount was observed after neoadjuvant chemotherapy and gastrectomy, while FASN and CD44 mRNA levels decreased only after gastrectomy. The amount of RNA and the relative mRNA levels of FASN and CD44 in EVs were affected more significantly by chemotherapy and gastrectomy than by chemotherapy alone. Therefore, they are a potential biomarker for monitoring treatment response. Future analyses are needed to identify GC-specific key RNAs in EVs, which could be used for the diagnosis of gastric cancer patients in order to determine their molecular subtype and to accompany the therapeutic response. Keywords: extracellular vesicle (EV); fatty acid synthase (FASN); gastric cancer; mRNA. Grant support

参考サイト PubMed: exsome



バイオクイックニュース日本語版:エクソソーム特集

バイオクイックニュース日本語版
10月 10, 2021 bioassociates2

エクソソームを用いたHIV治療の研究で成果。マウスにおいて骨髄、脾臓、脳内のHIVレベルが低下することが判明。

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