Characterization and proteomics of chicken seminal plasma extracellular vesicles


Posted: 2021-10-30 19:00:00
Reprod Domest Anim . 2021 Oct 30. doi: 10.1111/rda.14033. Online ahead of print. Affiliations Expand Affiliations 1 Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education. 2 College of Animal Science and Technology and College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China. Item in Clipboard Xuliang Luo et al. Reprod Domest Anim. 2021. Show details Display options Display options Format Reprod Domest Anim . 2021 Oct 30. doi: 10.1111/rda.14033. Online ahead of print. Affiliations 1 Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education. 2 College of Animal Science and Technology and College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China. Item in Clipboard CiteDisplay options Display options Format Abstract In mammals, seminal plasma extracellular vesicles (SPEVs) can regulate sperm motility and capacitation. The characteristics and functions of SPEVs in avians have been rarely reported. In this study, chicken SPEVs were isolated and characterized by transmission and scanning electron microscopy (TEM/SEM) and nanoparticle tracking analysis (NTA); furthermore, seven extracellular vesicles (EVs) marker proteins were detected by western blot (WB). TEM revealed that chicken SPEVs had a classic bilayer membrane structure. NTA confirmed that the size of SPEVs was 30 ~ 250 nm and concentration ranged from 8.0 E + 11 ~ 8.5 E +11 particles/ml. There were 3073 SPEVs proteins identified by deep sequencing, including 2794 intracellular proteins and 279 extracellular proteins. The overlap rate of proteomes between chicken SPEVs and vesicles reported in the Vesiclepedia database reached 86%, and 360 new proteins that had not been reported by the ExoCarta and Vesiclepedia databases were identified in chicken SPEVs proteomes. Gene Ontology (GO) analysis revealed that chicken SPEVs proteins were mainly enriched in supplying energy and transporting protein. There were 4 IFT family proteins speculated to play an important role in sperm composition and function. Our data were compared with two previously published studies on the proteomics of chicken seminal plasma (SP) and hen uterine fluid, some overlapping proteins described in chicken SPEVs had been identified in hen uterine fluid (545) and chicken SP (284). In conclusion, these findings will increase our understanding of the content and composition of proteome in SPEVs and provide new insights into the important role of the SPEVs regulation in sperm functions. Keywords: chicken; extracellular vesicles; proteome; seminal plasma. This article is protected by copyright. All rights reserved. LinkOut - more resources Research Materials [x] Cite Copy Format: Send To [x]

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バイオクイックニュース日本語版
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