Characterization and profiling analysis of bovine oviduct and uterine extracellular vesicles and their miRNA cargo through the estrous cycle

Posted: 2021-11-03 19:00:00
Extracellular vesicles (EVs) found in various biological fluids and particularly in reproductive fluids, have gained considerable attention for their possible role in cell- to- cell communication. Among, the different bioactive molecules cargos of EVs, MicroRNAs (miRNAs) are emerging as promising diagnostic biomarkers with high clinical potential. Aiming to understand the roles of EVs in bovine reproductive tract, we intended to characterize and profile the EVs of oviduct and uterine fluids (OF-EVs, UF-EVs) and their miRNA across the estrous cycle. Nanoparticle tracking analysis and transmission electron microscopy confirmed the existence of small EV population in OF and UF at all stages, (size between 30 and 200 nm; concentration: 3.4 × 1010 EVs/ml and 6.0 × 1010 EVs/ml for OF and UF, respectively, regardless of stage). The identification of EV markers (CD9, HSP70, and ALIX proteins) was confirmed by western blot. The miRNA analysis revealed the abundance of 310 and 351 miRNAs in OF-EVs and UF-EVs, respectively. Nine miRNAs were differentially abundant in OF-EVs between stages of the cycle, eight of them displayed a progressive increase from S1 to S4 (p < .05). In UF-EVs, a total of 14 miRNAs were differentially abundant between stages. Greater differences were observed between stage 1 (S1) and stage 3 (S3), with 11 miRNAs enriched in S3 compared to S1. Functional enrichment analysis revealed the involvement of these miRNAs in relevant pathways such as cell signaling, intercellular junctions, and reproductive functions that may be implicated in oviduct and uterus modulation across the cycle, but also in their preparation for embryo/conceptus presence and development. Keywords: estrous cycle; extracellular vesicles; microRNA; oviduct; uterus.

参考サイト PubMed: exsome


2月 07, 2019 バイオアソシエイツ


アラバマ大学バーミンガム校(University of Alabama at Birmingham :UAB)の研究者らは、慢性閉塞性肺疾患(COPD)患者の肺における慢性炎症と組織破壊との間の根本的なつながりである、これまで報告されていない新しい病原体を発見した。 COPDは世界における死因の第4位だ。精製された活性化多形核白血球(PMN: polymorphonuclear leukocytes)から収集された小さな細胞内粒子が健康なマウスの肺に点滴注入されたとき、この病原体 - PMN由来 エクソソーム -…

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