Bacterial membrane vesicles and phages in blood after consumption of lacticaseibacillus rhamnosus JB-1


Posted: 2021-11-08 20:00:00
Gut Microbes . Jan-Dec 2021;13(1):1993583. doi: 10.1080/19490976.2021.1993583. Affiliations Expand Affiliations 1 Neuroscience Graduate Program, McMaster University, Hamilton, Canada. 2 Brain-Body Institute, St. Joseph's Healthcare Hamilton, Hamilton, Canada. 3 Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Canada. 4 Farncombe Family Digestive Health Research Institute, Department of Medicine, McMaster University, Hamilton, Canada. 5 Department of Medicine, McMaster University, Hamilton, Canada. 6 Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Canada. Item in Clipboard Kevin Champagne-Jorgensen et al. Gut Microbes. Jan-Dec 2021. Show details Display options Display options Format Gut Microbes . Jan-Dec 2021;13(1):1993583. doi: 10.1080/19490976.2021.1993583. Affiliations 1 Neuroscience Graduate Program, McMaster University, Hamilton, Canada. 2 Brain-Body Institute, St. Joseph's Healthcare Hamilton, Hamilton, Canada. 3 Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Canada. 4 Farncombe Family Digestive Health Research Institute, Department of Medicine, McMaster University, Hamilton, Canada. 5 Department of Medicine, McMaster University, Hamilton, Canada. 6 Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Canada. Item in Clipboard CiteDisplay options Display options Format Abstract Gut microbiota have myriad roles in host physiology, development, and immunity. Though confined to the intestinal lumen by the epithelia, microbes influence distal systems via poorly characterized mechanisms. Recent work has considered the role of extracellular vesicles in interspecies communication, but whether they are involved in systemic microbe-host interaction is unclear. Here, we show that distinctive nanoparticles can be isolated from mouse blood within 2.5 h of consuming Lacticaseibacillus rhamnosus JB-1. In contrast to blood nanoparticles from saline-fed mice, they reproduced lipoteichoic acid-mediated immune functions of the original bacteria, including activation of TLR2 and increased IL-10 expression by dendritic cells. Like the fed bacteria, they also reduced IL-8 induced by TNF in an intestinal epithelial cell line. Though enriched for host neuronal proteins, these isolated nanoparticles also contained proteins and viral (phage) DNA of fed bacterial origin. Our data strongly suggest that oral consumption of live bacteria rapidly leads to circulation of their membrane vesicles and phages and demonstrate a nanoparticulate pathway whereby beneficial bacteria and probiotics may systemically affect their hosts. Keywords: Bacteriophage; TLR2; commensal; extracellular vesicles; interleukin 10; lactobacillus; microbiome; microbiota-gut-brain axis; microvesicle; probiotic. LinkOut - more resources Research Materials

参考サイト PubMed: exsome



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