A standarized protocol for evaluation of large extracellular vesicles using the attune NXT system


Posted: 2021-10-18 19:00:00
J Immunol Methods . 2021 Oct 15;113170. doi: 10.1016/j.jim.2021.113170. Online ahead of print. Affiliations Expand Affiliations 1 Dobney Hypertension Centre, School of Medicine - Royal Perth Hospital Unit, Medical Research Foundation, Faculty of Medicine, Dentistry & Health Sciences, The University of Western Australia, Perth, Australia. 2 Kidney Research Centre, The Ottawa Hospital Research Institute, Department of Cellular and Molecular Medicine, University of Ottawa, Canada. 3 Centre for Clinical Research in Emergency Medicine, Harry Perkins Institute of Medical Research, Perth, Western Australia, Australia. 4 Dobney Hypertension Centre, School of Medicine - Royal Perth Hospital Unit, Medical Research Foundation, Faculty of Medicine, Dentistry & Health Sciences, The University of Western Australia, Perth, Australia; Department of Internal Medicine, Royal Perth Hospital, Perth, Western Australia, Australia; Departments of Cardiology and Nephrology, Royal Perth Hospital, Perth, Australia; Dobney Hypertension Centre, School of Medicine - Royal Perth Hospital Unit, Medical Research Foundation, Faculty of Medicine, Dentistry & Health Sciences, The University of Western Australia, Perth, Australia. Electronic address: markus.schlaich@uwa.edu.au. Item in Clipboard Leslie Marisol Lugo-Gavidia et al. J Immunol Methods. 2021. Show details Display options Display options Format J Immunol Methods . 2021 Oct 15;113170. doi: 10.1016/j.jim.2021.113170. Online ahead of print. Affiliations 1 Dobney Hypertension Centre, School of Medicine - Royal Perth Hospital Unit, Medical Research Foundation, Faculty of Medicine, Dentistry & Health Sciences, The University of Western Australia, Perth, Australia. 2 Kidney Research Centre, The Ottawa Hospital Research Institute, Department of Cellular and Molecular Medicine, University of Ottawa, Canada. 3 Centre for Clinical Research in Emergency Medicine, Harry Perkins Institute of Medical Research, Perth, Western Australia, Australia. 4 Dobney Hypertension Centre, School of Medicine - Royal Perth Hospital Unit, Medical Research Foundation, Faculty of Medicine, Dentistry & Health Sciences, The University of Western Australia, Perth, Australia; Department of Internal Medicine, Royal Perth Hospital, Perth, Western Australia, Australia; Departments of Cardiology and Nephrology, Royal Perth Hospital, Perth, Australia; Dobney Hypertension Centre, School of Medicine - Royal Perth Hospital Unit, Medical Research Foundation, Faculty of Medicine, Dentistry & Health Sciences, The University of Western Australia, Perth, Australia. Electronic address: markus.schlaich@uwa.edu.au. Item in Clipboard CiteDisplay options Display options Format Abstract Extracellular vesicles (EVs) represent a promising biomarker source in several medical areas. Flow cytometry (FC) is one of the most widely-used methods to characterize EVs, providing quantitative information and determination of EV subtypes. EV evaluation represents a challenge as no standardized methods are available to facilitate assessment across different research centers. This is principally because their size falls below the detection limit of most standard flow cytometers and a thorough optimization process must be done to ensure instrument-specific sensitivity. We provide an overview of a standardized method to evaluate large EVs using the Attune™ Nxt Acoustic Focusing Flow Cytometer system (ThermoFisher Scientific). Keywords: Attune Nxt; Extracellular vesicles; Flow cytometry. Copyright © 2021. Published by Elsevier B.V. [x] Cite Copy Format: Send To [x]

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