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A Novel Silicon Platform for Selective Isolation, Quantification, and Molecular Analysis of Small Extracellular Vesicles


Posted: 2021-10-06 19:00:00
Introduction: Small extracellular vesicles (sEVs), thanks to their cargo, are involved in cellular communication and play important roles in cell proliferation, growth, differentiation, apoptosis, stemness and embryo development. Their contribution to human pathology has been widely demonstrated and they are emerging as strategic biomarkers of cancer, neurodegenerative and cardiovascular diseases, and as potential targets for therapeutic intervention. However, the use of sEVs for medical applications is still limited due to the selectivity and sensitivity limits of the commonly applied approaches. Methods: Novel sensing solutions based on nanomaterials are arising as strategic tools able to surpass traditional sensor limits. Among these, Si nanowires (Si NWs), realized with cost-effective industrially compatible metal-assisted chemical etching, are perfect candidates for sEV detection. Results: In this paper, the realization of a selective sensor able to isolate, concentrate and quantify specific vesicle populations, from minimal volumes of biofluid, is presented. In particular, this Si NW platform has a detection limit of about 2×105 sEVs/mL and was tested with follicular fluid and blastocoel samples. Moreover, the possibility to detach the selectively isolated sEVs allowing further analyses with other approaches was demonstrated by SEM analysis and several PCRs performed on the RNA content of the detached sEVs. Discussion: This platform overcomes the limit of detection of traditional methods and, most importantly, preserves the biological content of sEVs, opening the route toward a reliable liquid biopsy analysis. Keywords: biosensor; label-free; luminescence; silicon nanowires; small extracellular vesicles.

参考サイト PubMed: exsome



バイオクイックニュース日本語版:エクソソーム特集

バイオクイックニュース日本語版
2月 12, 2015 バイオアソシエイツ

新開発「Lab on a Chip」エクソソーム解析でがん早期発見に一大変革も

科学者は、「 エクソソーム 」と呼ばれる小胞に含まれる新しいバイオマーカーを使ってがんその他様々な疾患を早期発見する方法を見つけるために骨折ってきた。事実、「Popular Science」誌は、今年 (2014年) に世界を画することになる20の科学的業績の一つとしてエクソソーム・ベースのがん診断を挙げている。エクソソームは、より侵襲性の低いがん早期発見法につながり、がん患者の生存率を一気に引き上げる可能性がある。University of Kansasの化学准教授、Dr. Yong…

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