SARS-CoV-2 triggers DNA damage response in Vero E6 cells


Posted: 2021-10-02 19:00:00
Biochem Biophys Res Commun . 2021 Sep 15;579:141-145. doi: 10.1016/j.bbrc.2021.09.024. Online ahead of print. Affiliations Expand Affiliations 1 Department of Microbiology and Molecular Genetics, Larner College of Medicine, University of Vermont, Burlington, VT, 05405, USA. 2 Division of Immunobiology, Department of Medicine, Larner College of Medicine, University of Vermont, Burlington, VT, 05405, USA. 3 Department of Biochemistry, Duke University School of Medicine, Durham, NC, 27710, USA. 4 Department of Microbiology and Molecular Genetics, Larner College of Medicine, University of Vermont, Burlington, VT, 05405, USA; Division of Immunobiology, Department of Medicine, Larner College of Medicine, University of Vermont, Burlington, VT, 05405, USA. 5 Department of Microbiology and Molecular Genetics, Larner College of Medicine, University of Vermont, Burlington, VT, 05405, USA; Division of Immunobiology, Department of Medicine, Larner College of Medicine, University of Vermont, Burlington, VT, 05405, USA; University of Vermont Cancer Center, University of Vermont, Burlington, VT, 05405, USA. Electronic address: nimrat.chatterjee@med.uvm.edu. Item in Clipboard Joshua Victor et al. Biochem Biophys Res Commun. 2021. Show details Display options Display options Format Biochem Biophys Res Commun . 2021 Sep 15;579:141-145. doi: 10.1016/j.bbrc.2021.09.024. Online ahead of print. Affiliations 1 Department of Microbiology and Molecular Genetics, Larner College of Medicine, University of Vermont, Burlington, VT, 05405, USA. 2 Division of Immunobiology, Department of Medicine, Larner College of Medicine, University of Vermont, Burlington, VT, 05405, USA. 3 Department of Biochemistry, Duke University School of Medicine, Durham, NC, 27710, USA. 4 Department of Microbiology and Molecular Genetics, Larner College of Medicine, University of Vermont, Burlington, VT, 05405, USA; Division of Immunobiology, Department of Medicine, Larner College of Medicine, University of Vermont, Burlington, VT, 05405, USA. 5 Department of Microbiology and Molecular Genetics, Larner College of Medicine, University of Vermont, Burlington, VT, 05405, USA; Division of Immunobiology, Department of Medicine, Larner College of Medicine, University of Vermont, Burlington, VT, 05405, USA; University of Vermont Cancer Center, University of Vermont, Burlington, VT, 05405, USA. Electronic address: nimrat.chatterjee@med.uvm.edu. Item in Clipboard Full text links CiteDisplay options Display options Format Abstract The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus responsible for the current COVID-19 pandemic and has now infected more than 200 million people with more than 4 million deaths globally. Recent data suggest that symptoms and general malaise may continue long after the infection has ended in recovered patients, suggesting that SARS-CoV-2 infection has profound consequences in the host cells. Here we report that SARS-CoV-2 infection can trigger a DNA damage response (DDR) in African green monkey kidney cells (Vero E6). We observed a transcriptional upregulation of the Ataxia telangiectasia and Rad3 related protein (ATR) in infected cells. In addition, we observed enhanced phosphorylation of CHK1, a downstream effector of the ATR DNA damage response, as well as H2AX. Strikingly, SARS-CoV-2 infection lowered the expression of TRF2 shelterin-protein complex, and reduced telomere lengths in infected Vero E6 cells. Thus, our observations suggest SARS-CoV-2 may have pathological consequences to host cells beyond evoking an immunopathogenic immune response. Keywords: DNA damage Response; Genome instability; SARS-CoV-2; Telomeres. Copyright © 2021 Elsevier Inc. All rights reserved. Conflict of interest statement Declaration of competing interest Authors declare no conflict of interest. LinkOut - more resources Full Text SourcesResearch MaterialsMiscellaneous [x] Cite Copy Format: Send To [x]

参考サイト PubMed: covid-19



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