Drug resistance in Candida albicans isolates and related changes in the structural domain of Mdr1 protein


Posted: 2021-11-19 20:00:00
Background: The increasing azole drug resistance in fungal pathogens poses a pressing threat to global health care. The coexistence of drug-resistant Candida albicans with tuberculosis patients and the failure of several drugs to treat C. albicans infection extend hospital stay, economic burden, and death. The misuse or abuse of azole-derived antifungals, chronic use of TB drugs, different immune-suppressive drugs, and diseases like HIV, COVID-19, etc., have aggravated the situation. So it is vital to understand the molecular changes in drug-resistant genes to modify the treatment to design an alternative mechanism. Method: C. albicans isolated from chronic tuberculosis patients were screened for antifungal sensitivity studies using disk diffusion assay. The multidrug-resistant C. albicans were further screened for molecular-level changes in drug resistance using MDR1 gene sequencing and compared with Gen bank data of similar species using the BLAST tool. Results: The investigation proved that the isolated C. albicans from TB patients are significantly resistant to the action of six drugs. The molecular changes in MDR1 genes showed differences in seven nucleotide base pairs that interfered with the efflux pump. Keywords: Candida spp.; Drug resistance; Fungi; MDR1.

参考サイト PubMed: covid-19



バイオクイックニュース日本語版:COVID-19特集

バイオクイックニュース日本語版
12月 21, 2020 バイオアソシエイツ

脂肪組織がCOVID-19の悪化に重要な役割を果たすという仮説

脂肪組織が COVID-19 の悪化に重要な役割を果たすという証拠が増えている。 調査中の理論の1つは、脂肪細胞(adipocytes)がSARS-CoV-2の貯蔵庫として機能し、肥満または太りすぎの人のウイルス量を増加させるというものだ。科学者らはまた、感染中に脂肪細胞が血流中に放出され、生体内のウイルスによって引き起こされる炎症反応を促進すると考えている。これらの仮説は、ブラジルのサンパウロ大学医学部(FM-USP)臨床外科の教授 Marilia Cerqueira Leite…

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