DNA-Directed Patterning for Versatile Validation and Characterization of a Lipid-Based Nanoparticle Model of SARS-CoV-2


Posted: 2021-10-21 19:00:00
Adv Sci (Weinh) . 2021 Oct 21;e2101166. doi: 10.1002/advs.202101166. Online ahead of print. Affiliations Expand Affiliations 1 California Institute for Quantitative Biosciences, University of California, Berkeley, 174 Stanley Hall, Berkeley, CA, 94720, USA. 2 UC Berkeley-UC San Francisco Graduate Program in Bioengineering, University of California, Berkeley, 306 Stanley Hall, Berkeley, CA, 94720, USA. 3 Department of Mechanical Engineering, University of California, Berkeley, 5118 Etcheverry Hall, Berkeley, CA, 94720, USA. Item in Clipboard Molly Kozminsky et al. Adv Sci (Weinh). 2021. Show details Display options Display options Format Adv Sci (Weinh) . 2021 Oct 21;e2101166. doi: 10.1002/advs.202101166. Online ahead of print. Affiliations 1 California Institute for Quantitative Biosciences, University of California, Berkeley, 174 Stanley Hall, Berkeley, CA, 94720, USA. 2 UC Berkeley-UC San Francisco Graduate Program in Bioengineering, University of California, Berkeley, 306 Stanley Hall, Berkeley, CA, 94720, USA. 3 Department of Mechanical Engineering, University of California, Berkeley, 5118 Etcheverry Hall, Berkeley, CA, 94720, USA. Item in Clipboard CiteDisplay options Display options Format Abstract Lipid-based nanoparticles have been applied extensively in drug delivery and vaccine strategies and are finding diverse applications in the coronavirus disease 2019 (COVID-19) pandemic-from vaccine-component encapsulation to modeling the virus, itself. High-throughput, highly flexible methods for characterization are of great benefit to the development of liposomes featuring surface proteins. DNA-directed patterning is one such method that offers versatility in immobilizing and segregating lipid-based nanoparticles for subsequent analysis. Here, oligonucleotides are selectively conjugated onto a glass substrate and then hybridized to complementary oligonucleotides tagged to liposomes, patterning them with great control and precision. The power of DNA-directed patterning is demonstrated by characterizing a novel recapitulative lipid-based nanoparticle model of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-S-liposomes-that presents the SARS-CoV-2 spike (S) protein on its surface. Patterning a mixture of S-liposomes and liposomes that display the tetraspanin CD63 to discrete regions of a substrate shows that angiotensin-converting enzyme 2 (ACE2) specifically binds to S-liposomes. Subsequent introduction of S-liposomes to ACE2-expressing cells tests the biological function of S-liposomes and shows agreement with DNA-directed patterning-based assays. Finally, multiplexed patterning of S-liposomes verifies the performance of commercially available neutralizing antibodies against the two S variants. Overall, DNA-directed patterning enables a wide variety of custom assays for the characterization of any lipid-based nanoparticle. Keywords: DNA-directed patterning; SARS-CoV-2; liposomes; neutralizing antibodies; spike. © 2021 The Authors. Advanced Science published by Wiley-VCH GmbH. References L. Sercombe, T. Veerati, F. Moheimani, S. Y. Wu, A. K. Sood, S. Hua, Front. Pharmacol. 2015, 6, 286. S. Vemuri, C. T. Rhodes, Pharm. Acta Helv. 1995, 70, 95. V. P. Torchilin, Nat. Rev. Drug Discovery 2005, 4, 145. M. L. Guevara, F. Persano, S. Persano, Front. Chem. 2020, 8, 589959. U. Bulbake, S. Doppalapudi, N. Kommineni, W. Khan, Pharmaceutics 2017, 9, 12. Show all 38 references Grant support Show all 6 grants [x] Cite Copy Format: Send To [x]

参考サイト PubMed: covid-19



バイオクイックニュース日本語版:COVID-19特集

ゲスト 842人 と メンバー 4人 がオンラインです