Discovery and initial characterization of YloC, a novel endoribonuclease in Bacillus subtilis


Posted: 2021-11-24 20:00:00
. 2021 Nov 23;rna.078962.121. doi: 10.1261/rna.078962.121. Online ahead of print. Affiliations Expand Affiliations 1 Icahn School of Medicine at Mount Sinai. 2 University of Pennsylvania. 3 National Cancer Institute. 4 Icahn School of Medicine at Mount Sinai; david.bechhofer@mssm.edu. Item in Clipboard Shakti Ingle et al. RNA. 2021. Show details Display options Display options Format . 2021 Nov 23;rna.078962.121. doi: 10.1261/rna.078962.121. Online ahead of print. Affiliations 1 Icahn School of Medicine at Mount Sinai. 2 University of Pennsylvania. 3 National Cancer Institute. 4 Icahn School of Medicine at Mount Sinai; david.bechhofer@mssm.edu. Item in Clipboard CiteDisplay options Display options Format Abstract Bacillus subtilis genome is predicted to encode numerous ribonucleases, including four 3' exoribonucleases that have been characterized to some extent. A strain containing gene knockouts of all four known 3' exoribonucleases is viable, suggesting that one or more additional RNases remain to be discovered. A protein extract from the quadruple RNase mutant strain was fractionated and RNase activity was followed, resulting in identification of an enzyme activity catalyzed by the YloC protein. YloC is an endoribonuclease and is a member of the highly conserved "YicC family" of proteins that is widespread in bacteria. YloC is a metal-dependent enzyme that catalyzes cleavage of single-stranded RNA, preferentially at U residues, and exists in an oligomeric form, most likely a hexamer. As such, YloC shares some characteristics with the SARS-CoV Nsp15 endoribonuclease. While the in vivo function of YloC in B. subtilis is yet to be determined, YloC was found to act similarly to YicC in an Escherichia coli in vivo assay that assesses decay of the small RNA, RyhB. Thus, YloC may play a role in small RNA regulation. Keywords: Bacillus subtilis; bacteria; endoribonuclease; oligomer; small RNA processing. Published by Cold Spring Harbor Laboratory Press for the RNA Society. [x] Cite Copy Format: Send To [x]

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